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- Reflect on your success with the gram stain. Provide a complete analysis of your stain technique and results. Did you have success with several different bacteria? If you had problems discuss them and your plan to improve your success next time.
- Rate your confidence from 1-5 (1=completely confident, 5=not very confident) on being able to accurately perform and assess a gram stain. If you are not very confident (3, 4, or 5), please see your instructor for help and a pep talk!
- Smith, Ann C., Hussey, Marise A. The Gram Stain, in Laboratory Protocols. ASM Microbe Library. July 2013 www.microbelibrary.org/component/resource/gram-stain/2886-gram-stain-protocols. Accessed 11/25/15
- Gram, Christian, The differential staining of Schizomycetes in tissue sections and in dried preparations. 1884. www.microbelibrary.org/images/stories/ML_2.0/1884p215.pdf. Accessed 12/11/15
Linux is a flexible, robust node operating system for Beowulf computational clusters. Stability and adaptability set it apart from the legacy operating systems that dominate desktop environments. While not a "cancer" like some detractors have labeled Linux, it has spread quickly from its humble beginnings as a student's hobby project to a full-featured server operating system with advanced features and legendary stability. And while almost any Linux distribution will perform adequately as a Beowulf node operating system, a little tuning and trimming will skinny down the already lean Linux kernel, leaving more compute resources for scientific applications. If this chapter seems a little overwhelming, we note that there are companies that will completely configure and deliver Beowulf systems, including all the aforementioned tweaks and modifications to the kernel. There are also revolutionary systems such as the Beowulf software from Scyld Computing Corporation (www.sycld.com). The software from Scyld combines a custom Linux kernel and distribution with a complete environment for submitting jobs and administering the cluster. With its extremely simple single-system image approach to management, the Scyld software can make Beowulfs very easy indeed. Chapter 18 is devoted to a discussion of the Scyld approach.
One final reminder is in order. Many Beowulf builders became acquainted with Linux purely out of necessity. They started constructing their Beowulf saying, "Every OS is pretty much like every other, and Linux is free. free is good, right?". On the back of restaurant napkins, they sketched out their improved price/performance ratios. After the hardware arrived, the obligatory LINPACK report was sent to the Top500 list, and the real scientific application ran endlessly on the new Beowulf. Then it happened. Scientists using Linux purely as a tool stopped and peered inquisitively at the tool. They read the source code for the kernel. Suddenly, the simulation of the impending collision of the Andromeda galaxy with our own Milky Way seemed less interesting. Even though the two galaxies are closing at a rate of 300,000 miles per hour and we have only 5 billion years to wait, the simulation simply seemed less exciting than improving the virtual memory paging algorithm in the kernel source, sending Linus Torvalds the patch, and reading all the kernel mailing list traffic. Beware. Even the shortest of peeks down the rabbit's hole can sometimes lead to a wonderland much more interesting than your own.
The global SARS-CoV-2 pandemic forced universities throughout the world to shift their missions to address this threat. Ultimately, their responses were largely successful and SARS-CoV-2 infections and spread were undoubtedly mitigated during the ensuing shutdowns. However, these responses were not always executed smoothly, in part because of the unparalleled nature of the pandemic. Because we may well face another SARS-CoV-2-associated shutdown, we must consider how effective these responses were. This Perspective’s goal is to generate a framework for these discussions and provide views of faculty members from the proverbial trenches. Although our perspectives are those of medical school faculty, we expect that many of the issues we observed will apply to other academic venues in the United States and throughout the world. In preparing to write this Perspective, we solicited opinions directly from colleagues as well as through social media (e.g. Twitter). Highlights of these communications will be presented below. They are redacted for confidentiality.
Initial SARS-CoV-2 responses focused on students since education and training are important missions of universities. There were two compelling reasons for this focus. First, the pandemic overlapped with many academic holidays (‘Spring Breaks’) when students leave their campuses, which increased their risk of SARS-CoV-2 exposure that could be spread across campuses upon their return. Second, students in academic health programs (e.g. medicine, dentistry, and nursing) perform clinical rotations, which could expose them and their potentially high-risk patients to SARS-COV-2. Thus, universities quickly and decisively cancelled in-person classes and training. Courses were delivered by video conferencing (e.g. WEBEX, Zoom) with the goal of protecting the institutions while transitioning to a “business as usual” model.
But there were hurdles and barriers. Many faculty members were not familiar with online conferencing software, lacked required tools (e.g. Webcams, high quality microphones), and received limited, if any, training in online content delivery. In addition, significant increases in online content stretched bandwidth capabilities of most institutes. While these issues were expected, others were less so. For example, abrupt dorm closures required students to find alternative housing with high-speed internet access to attend their classes. In addition, students (and faculty) with children or other dependents required homeschooling and alternative care plans that conflicted with classes they either were enrolled in or taught. Thus, socioeconomic disparities undoubtedly affected students’ opportunities and performances. Mental and physical health resources were also impacted, thereby preventing students, faculty, and staff from accessing necessary care and treatment. This was particularly acute given the adverse mental health consequences of the pandemic and subsequent lockdown.
Besides didactic classes, medical school clerkships and laboratory-based classes were also cancelled. Since these are often required for certification and license, cancellations had significant impacts on career progressions. Some schools provided web-based content and/or de-identified case presentations. But, it remains unclear how accreditation agencies will assess these substitutions.
Graduate student courses and presentations also were shifted to online formats. But requirements to work in their laboratories went unchanged. This contradiction laid bare a long-standing issue of how the role of graduate students in the research enterprise is defined–trainees vs. skilled workforce. In many cases, universities allowed individual faculty to determine their laboratory’s status (e.g. open, closed, minimally staffed) as well as to identify “essential” laboratory. In many cases, personnel and trainees were limited in contesting their mentors’ decisions.
Finally, the shutdown posed significant and specific challenges to foreign students. Dorm closures were often executed without housing solutions in place for those requiring alternatives and/or were ineffectively communicated. One option was to return home, but the rapid and changing landscape surrounding travel and visa policies made that option tenuous.
As universities recognized the pandemic’s dangers, they began plans to shut down research laboratories. Some staff moved offsite and focused on project planning, writing, and data analysis. But, the shutdown was not as straightforward for essential duties. For example, laboratory animals require continued care. In many cases, decisions and plans were enacted with key entities collaborating with the overarching goal of maintaining personnel safety. These directives were largely unilateral and delivered to faculty in unambiguous terms. Other swiftly implemented decisions included accommodating research groups who possessed expertise to work on SARS-CoV-2 while creating protocols such as social distancing and PPE use for their safety.
Feedback from social media, conversations with colleagues, and our own observations revealed that decision-making regarding “non-essential” research areas was less efficient and unilateral. There were three primary reasons for this: i) researchers who believed their work was essential despite tenuous relations to SARS-CoV-2 ii) others who had difficulty accepting that their work was not essential and iii) ambiguous directives from local, state, and federal agencies that were left open to interpretation. For example, the State of Pennsylvania’s edict that Agriculture is an essential industry was interpreted to mean that non COVID-19 related agricultural research was also essential and could continue while non COVID-19 medical research could not . Institutes needed to review petitions and decide which laboratories remained open and often did so under pressure from PIs. In rare cases, investigators ignored shutdown orders and continued laboratory operations as normal. These instances revealed the difficulties universities face in enforcing their own directives.
Clear communication from university leadership was often lacking. For example, one coauthor’s institution remained largely silent until well past SARS-CoV-2 was designated a global pandemic. In addition, messaging from the other co-authors’ universities, and those of colleagues who communicated via social media, was mixed and unclear. Indeed, of 200 respondents to a Twitter poll, >48% indicated that institutional guidance was lacking (https://twitter.com/scienceCC/status/1245016170606088192). Many investigators were confused by which guidelines were being implemented, delaying their ability to communicate directives to their research groups. This led to laboratory staff being required to work without clear mandates and/or mechanisms to be excused. This point is critical, as power dynamics within academic laboratories put staff and trainees in situations where retribution is possible.
Finally, damage to career trajectories was felt at almost every level. Students’ and fellows’ progression towards completing their studies were halted as was faculty career development, which disproportionately affected junior faculty. While most institutes extended tenure clocks, other issues have yet to be resolved and/or addressed including how to recoup funding lost or lapsed during the shutdown.
Power structures at universities as a barrier to crisis management
Almost every academic health center faced identical circumstances as SARS-CoV-2 spread across the country. University leaders were in the unenvious position of making critical decisions based on rapidly evolving information. While employee and student safety was of paramount concern, compliance with accrediting agencies and integration with hospital partners needed to be considered. In addition, decisions needed to align with local, state, and national government shutdown orders. Compounding this was a lack of testing capabilities that led to uncertainty about infection prevalence.
All of these factors challenged how impactful decisions are made in universities. Effective university leaders are accustomed to making major decisions after input from various stakeholders and through shared-governance. Under routine circumstances this system benefits everyone—faculty are heard and leaders are well aware of a decision’s “buy-in” before its announcement. Academic institutions, by their very nature, do not hire leaders for their ability to make fast and difficult decisions in acute disasters or crises, as these are rare events. Thus, sweeping pronouncements from university leaders are uncommon. In contrast, effective leaders during a crisis are those who can make unilateral decisions, communicate them clearly and unambiguously, and motivate people to execute their plans. In our opinion, the skillsets that make university leaders effective under normal circumstances could very well be an impediment in a time of crisis because many lack the proficiencies and experience to guide their institutions’ responses to catastrophic events.
The shutdown also exposed weaknesses in university leadership structures. These included expansive administrative structures of many institutions, which complicated the process of identifying and mobilizing key leaders. In addition, there were instances of “death by committee”, whereby leaders created committees, which delayed responses by diverting precious time and resources to the task of assembling, organizing, and empowering them. Thus, as this pandemic continues, and other crises arise, universities must reconsider whether their leadership is equipped and has the requisite skillsets to manage their institutions during crises.
Our aim is to develop a framework for leadership and faculty to assess their responses. We recognize that others may have opposing opinions and/or additional suggestions. Our hope is to initiate discussions so that clear and effective plans are in place before future crises arise. Specific recommendations are discussed below.
- Crisis management team preparation, training, and membership:
We propose that, if they have not done so already, individuals (e.g. Presidents, Provosts, and Deans) with the ultimate responsibility for making and implementing action plans undergo crisis management training. In addition, rosters for task forces and advisory groups responsible for helping to draft these plans should be in place before a crisis arises. Importantly, membership should be diverse and include those of all academic ranks and include equality of sex and race. During SARS-CoV-2, the gender and race disparities that exist in medical schools were unfortunately reflected in these committees. Committees and working groups should also include mental health experts, so that university leadership can access their expertise as they develop and execute plans. Finally, a single person/entity is needed to coordinate these efforts and who has the centralized authority, resources, and unilateral responsibility to put response plans into action.
Universities need to develop and distribute situational criteria to determine when classes should be moved online. Second, accommodations are required to enable students to participate in delivery of online learning sessions. This includes recording online sessions to enable students to hear lectures missed due to non-academic obligations. In addition, financial consequences of such decisions must be considered and aid provided to students and faculty for purchasing necessary equipment. Third, students must recognize that faculty will likely accrue responsibilities (e.g. homeschooling) and that flexibility is needed in scheduling lectures and meetings. Similarly, departmental and college leaders need to have frank conversations with their faculty and provide necessary resources. Open lines of communication are essential such that faculty can discuss struggles without fear of being viewed negatively.
Besides didactic lectures, journal clubs, student talks, qualifying exams, thesis committee meetings also began taking place online. While some (e.g. final defenses) are urgent, others are less so. Prioritizing online sessions is important as “burn-out” from online sessions is emerging as a problem . We therefore recommend that priorities be established before the next shutdown so that faculty and students know of and understand expectations.
Regardless of whether they are freshman, graduate students, postdoctoral fellows, or medical residents, commencing studies is stressful under normal circumstances. To alleviate this stress and anxiety, many schools use programs that promote a sense of community within each cohort group. Thus, the impact of policies such as social distancing and online education on new student acclimation will need to be considered. This could be accomplished by holding regular “virtual happy hours” and town hall-type meetings to facilitate social engagement between incoming and existing students.
- Essential versus non-essential research designations:
It is critical that universities establish clear procedures and protocols for defining essential research. Clarity would mitigate the adverse impacts on morale that many investigators felt after suspending their non-essential research, only to observe others not doing the same. Ideally, these distinctions would occur while avoiding conflicts of interest (e.g. not allowing faculty to make decisions about their own research). In addition, institutions need to consider the needs of laboratory staff and trainees and include them in the decision-making process.
- Human resources/career development:
The lessons learned from this shutdown should become part of any institution’s future SOPs. This includes essential personnel designation, telecommuting rules and regulation, and ensuring uninterrupted salary disbursements. It would be critical to engage laboratory staff and trainees in these protocols, particularly in defining who is essential and what mechanisms are in place to appeal these designations.
Although many schools created contingency plans to provide tenure extensions, they were announced only after junior faculty felt significant angst and concern. Thus, tenure and promotion bylaws should be modified with specific rules that would be automatically implemented in case of future shutdowns. Mechanisms are also needed to support faculty whose funding will lapse or be delayed due to a shutdown. These are obviously difficult decisions since different institutes have different levels of endowments and savings and unique rules for utilizing them. But we believe that institutions are obliged to limit impacts on individual faculty, trainees, and staff. Transparency is also essential in planning how to tackle financial shortcomings.
Shutdown plans must emphasize mitigating consequences on vulnerable populations. For example, many women with children often became primary caregivers, which undoubtedly will delay career progressions. Institutions (and funding agencies) must address these disparities so that an already leaky pipeline isn’t exacerbated. Finally, those most impacted by these imbalances should be partners in developing these plans and mechanisms created to provide, in real time, feedback so that corrections can be made.
Institutional leaders must provide updates regarding the status of the shutdown process, even if those updates are merely “we are working on it”. These communications must also be delivered with a single voice, as conflicting messages create tension and confusion. Because of time demands put on leaders during a crisis, we recommend creating a crisis communications team that is directed by and answers to the highest academic officer (e.g. university president or provost). Faculty, staff, and trainees should be encouraged to ask questions, even if it means going outside of the “chain of command”. Finally, institutions should appoint pandemic ombudsmen to confidentially address individual complaints and concerns.
19.6: Conclusion and Resources - Biology
New International Version
He told them, “Consider carefully what you do, because you are not judging for mere mortals but for the LORD, who is with you whenever you give a verdict.
New Living Translation
and he said to them, “Always think carefully before pronouncing judgment. Remember that you do not judge to please people but to please the LORD. He will be with you when you render the verdict in each case.
English Standard Version
and said to the judges, “Consider what you do, for you judge not for man but for the LORD. He is with you in giving judgment.
Berean Study Bible
Then he said to the judges, “Consider carefully what you do, for you are not judging for man, but for the LORD, who is with you when you render judgment.
King James Bible
And said to the judges, Take heed what ye do: for ye judge not for man, but for the LORD, who is with you in the judgment.
New King James Version
and said to the judges, “Take heed to what you are doing, for you do not judge for man but for the LORD, who is with you in the judgment.
New American Standard Bible
He said to the judges, “Consider what you are doing, for you do not judge for mankind but for the LORD who is with you when you render judgment.
He said to the judges, “Consider what you are doing, for you do not judge for man but for the LORD who is with you when you render judgment.
And he said to the judges, “Consider what you are doing, for you do not judge for man but for the LORD who is with you when you render judgment.
and he said to the judges, “Be careful what you do, for you do not judge for man, but for the LORD who is with you in the matter of judgment.
Christian Standard Bible
Then he said to the judges, “Consider what you are doing, for you do not judge for a man, but for the LORD, who is with you in the matter of judgment.
Holman Christian Standard Bible
Then he said to the judges, “Consider what you are doing, for you do not judge for man, but for the LORD, who is with you in the matter of judgment.
American Standard Version
and said to the judges, Consider what ye do: for ye judge not for man, but for Jehovah and he is with you in the judgment.
Aramaic Bible in Plain English
And he said to the Judges: “See what you do, that it is not for the children of men you judge, but for LORD JEHOVAH our God, and be strong and judge the judgment of truth, and LORD JEHOVAH will be with you for eternity
Brenton Septuagint Translation
And he said to the judges, Take good heed what ye do: for ye judge not for man, but for the Lord, and with you are matters of judgment.
Contemporary English Version
and told them: Be careful when you make your decisions in court, because you are judging by the LORD's standards and not by human standards, and he will know what you decide.
And charging the judges, he said: Take heed what you do: for you exercise not the judgment of man, but of the Lord: and whatsoever you judge, it shall redound to you.
English Revised Version
and said to the judges, Consider what ye do: for ye judge not for man, but for the LORD and he is with you in the judgment.
Good News Translation
and instructed them: "Be careful in pronouncing judgment you are not acting on human authority, but on the authority of the LORD, and he is with you when you pass sentence.
GOD'S WORD® Translation
He told the judges, "Pay attention to what you're doing. When you judge, you aren't doing it for a human but for the LORD. He will be with you when you hear a case.
International Standard Version
"Pay careful attention to your duties, because you are judging not only for the sake of human beings but also for the Lord—and he is present with you as you make your rulings.
JPS Tanakh 1917
and said to the judges: 'Consider what ye do for ye judge not for man, but for the LORD and [He is] with you in giving judgment.
Literal Standard Version
and says to the ones judging, “See what you are doing—for you do not judge for man, but for YHWH, who [is] with you in the matter of judgment
He told the judges, "Be careful what you do, for you are not judging for men, but for the LORD, who will be with you when you make judicial decisions.
New Heart English Bible
and said to the judges, "Consider what you do: for you do not judge for man, but for the LORD and he is with you in the judgment.
World English Bible
and said to the judges, "Consider what you do: for you don't judge for man, but for Yahweh and [he is] with you in the judgment.
Young's Literal Translation
and saith unto the judges, 'See what ye are doing -- for not for man do ye judge, but for Jehovah, who is with you in the matter of judgment
You must not pervert justice you must not show partiality to the poor or favoritism to the rich you are to judge your neighbor fairly.
Show no partiality in judging hear both small and great alike. Do not be intimidated by anyone, for judgment belongs to God. And bring to me any case too difficult for you, and I will hear it."
God presides in the divine assembly He renders judgment among the gods:
And said to the judges, Take heed what you do: for you judge not for man, but for the LORD, who is with you in the judgment.
Joshua 22:5 But take diligent heed to do the commandment and the law, which Moses the servant of the LORD charged you, to love the LORD your God, and to walk in all his ways, and to keep his commandments, and to cleave unto him, and to serve him with all your heart and with all your soul.
1 Chronicles 28:10 Take heed now for the LORD hath chosen thee to build an house for the sanctuary: be strong, and do it.
Luke 12:15 And he said unto them, Take heed, and beware of covetousness: for a man's life consisteth not in the abundance of the things which he possesseth.
Deuteronomy 1:17 Ye shall not respect persons in judgment but ye shall hear the small as well as the great ye shall not be afraid of the face of man for the judgment is God's: and the cause that is too hard for you, bring it unto me, and I will hear it.
Psalm 82:1-6 A Psalm of Asaph. God standeth in the congregation of the mighty he judgeth among the gods…
Ecclesiastes 5:8 If thou seest the oppression of the poor, and violent perverting of judgment and justice in a province, marvel not at the matter: for he that is higher than the highest regardeth and there be higher than they.